Drying the graduated cylinders better. However if the cells have a small surface area then the reaction rate will be slow because less of the enzyme will be accessible to the product and therefore fewer products will be made in a certain amount of time. Concentration of hydrogen peroxide: 2% of hydrogen peroxide was used through all trials this is prepared by adding 20cm3 of H2o2 to 1000cm3 of water. More appropriate sized graduated cylinders that are closer to the amount of mL of space we required. In this catalase and hydrogen peroxide experiment, we will discover how enzymes act as catalysts by causing chemical reactions to occur more quickly within living things.
Moreover, enzymes are specific in action. This couldVe been for a number of reasons. If the human body was to suffer from a case of acidosis where the body became to acidic it would negatively affect the decomposition of hydrogen peroxide by slowing do the process. I can tell that this is true form my graph because the line for the liver reaction rate is steeper that the line for the potato reaction rate. Repeat for the remaining pH levels. I think that grinding the materials will increase enzyme activity.
What gas is being released? So therefore I predict that the reaction would be faster then the cut in half potato. The longest reaction was for the pH 2. When molecules move fast enough they collide into one another, allowing chemical reactions to occur. To eliminate human error in the experiment the measurements and timing would have to be done by using more technological equipment, in order to gain precise results. A potato cutter that cut the potato into equal sizes. Hypothesis: If the peroxidase enzyme is reacting with potatoes at temperatures extremely high, the reaction will become denatured causing no reaction to occur. Then I measured out 20 ml of hydrogen peroxide and diluted it with 20 ml of water making a 50% concentration solution of hydrogen peroxide.
Be specific in your description, use drawings if necessary. Materials: Potato Test tube or other small container Hydrogen Peroxide 1. In order to make an enzyme, hundreds of amino acids are strung together in a very specific and unique order and eventually is folded. The experiment was also made a fair test because I used the same top pan scales each time I did the experiment. We performed five trials of this. When the experiment start a Suck 5 ml hydrogen peroxide by the syringe. First of all I tried to measure the reaction rate of 5g of crushed potato in 20 mls of hydrogen peroxide diluted with 20 mls of water.
Catalase, Catalysis, Chemical reaction 809 Words 3 Pages The effect of enzyme concentration, substrate concentration, pH, and temperature on the enzyme catalase. Remove the test tube from the hot water bath, allow it to air cool, then pour out the water. The oxygen can be observed as bubbles coming from the reaction site. These reactions allow the cell to build things or take things apart as needed. I tried the experiment with only 2g of crushed liver and I used a bigger beaker to contain the reaction better. Less foam means it is not working as well, and no foam means it is not working at all. I also used gloves, safety goggles and a lab coat to make sure that I was protected.
A Simple Catalase Experiment Using Potatoes: Some plants such as potato and spinach have very high levels of catalase, far higher they they would likely ever need to prevent photorespiration damage. Conclusion: The enzyme catalase is a common enzyme in animal and plant tissues. This principle is based on something called the collision theory. Why that is, no one seems to know. According to our results in table 2 and graph, the optimum temp was 37c? This reaction is caused by catalase, an enzyme within the potato. Our results showed that as the concentration of H2O2 increased, the rate of reaction increased. Catalase is an enzyme that catalyzes the transformation of hydrogen peroxide into water and oxygen.
But there are certain conditions that need to be fulfilled in order for. If changed, then this would affect the rate of enzyme activity, therefore affecting the results. Catalase, Catalysis, Chemical reaction 1505 Words 9 Pages Experiments investigating the effect of a particular factor on an enzyme-catalysed reaction are commonly used as assessed practicals in Biology. Catalase is an enzyme that brings about the reaction by which hydrogen peroxide is decomposed to water and oxygen Encyclopedia Britannica. Surpassing the optimum temperature will result in a drop in enzyme activity. The enzyme is not altered by the reaction. Our data shows a linear trend, however, research shows that the trend is in fact a parabola opening downwards with a maximum at a pH of 5, the acidity of Hydrogen Peroxide alone.
This caused a draft around my experiment and it changed my results. If I were to do the experiment again I would try to do it in a place with less people. Volume of oxygen bubbles cm3 Volume of cylinder:? Alternate Hypothesis 2: If there is an increase in concentration in concentration of Hydrogen Peroxide then the reaction rate of the liver catalase will decrease. Also, they can speed up Metabolic reactions without being changed or used up. Enzymes are proteins that speed up the rate of reactions that would otherwise happen more slowly. The results obtained matched the hypothesis which stated that 37c? Without the presence of enzymes some of life's processes would not come so easily. Factors such as temperature and concentrations can either help increase or decrease these reactions.
Biological catalysts are enzymes produced by living things, the potato is therefore is an enzyme the chemical reaction in the living cells are quite fast in condition which are warm, so therefore the potato would be best at a warm temperature rather then a hot one. A reaction will take place its products are water and oxygen. Similarly when the solution becomes more basic the rate decreases as we can see in trial two which yields a pH of 8. If changed, whether decrease or increase, then the number of enzymes active site available would change, therefore affecting the rate of the reaction. For instance I could see if the potato skin had more catalase in it then potato flesh does.